X-Ray Crystallography

Head:    Marcus Hartmann
Staff:   Reinhard Albrecht, Kerstin Baer
Phone:   + 49 7071 601-323


The primary goal of structural biology is a mechanistic understanding of biological macromolecules and of biological processes so that they can be described in the language of physics and chemistry. This requires an exact knowledge of the three-dimensional structures of the respective molecules and of their complexes such that the spatial position of each atom can be precisely described. Since the pioneering work of Max Perutz and Sir John Kendrew X-ray crystallography has been the single most important technique for the de­termination of three-dimensional protein structures. Currently, the X-ray coordinates for more than 50,000 macromolecules can be found in the protein database (PDB), far exceeding any other comparable technique. To obtain a crystal structure of a macromolecule one needs to purify several hundred micrograms of material in soluble form and one needs to obtain crystals that can be as small as a few micrometers in diameter. These crystals are then exposed to diffract a finely tuned monochromatic X-ray beam, nowadays typically generated by a synchrotron cyclic particle accelerator such as the Swiss Light Source, where the Max Planck Soci­ety partially owns one of the beamlines (PX II). Once diffraction data are recorded, electron density throughout the crystal can be computed and macromolecular models can be built.

Structural Biology of mRNA Localization Fulvia Bono
Molecular Recognition and Catalysis Marcus Hartmann
Protein Design Birte Höcker
Post-transcriptional Regulation of mRNAElisa Izaurralde
Protein Evolution Andrei Lupas
Large Molecular Complexes in mRNA Degradation Remco Sprangers
Retrotransposition and Regulatory RNAs Oliver Weichenrieder
Mechanism of Ubiquitin-dependent Cell Signaling Silke Wiesner

Genomic Solutions Honeybee 963 Crystallization Robot
TTP Labtech Mosquito Crystallization Robot
TTP Labtech Dragonfly Screen Optimisation Robot
Dornier LTF PIRO Pipetting Robot
Formulatrix Rock Imager 54 Imaging System @ 8 °C
Formulatrix Rock Imager 182 Imaging System with UV optics @ 20 °C

Xenocs sealed Tube X-ray Generator
Oxford Cryosystems Cryostream 700
MarResearch MAR345 Image Plate Detector
Regular access to beamline X10SA at the SLS with PILATUS 6M Detector